DYRK2, His-Tag Recombinant

Catalog #

40053

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human DYRK2 (dual specificity tyrosine phosphorylation regulated kinase 2), encompassing amino acids 2-528 (end). This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

dual specificity tyrosine phosphorylation regulated kinase 2, DYRK-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

DYRK2 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

DYRK2 (His-2-528(end))

Host Species/Expression System

Sf9

Purity

≥52%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol and 3mM DTT

64 kDa

Amino Acids

2-528(end)

Specific Activity

300 pmoles/min/μg

Genbank #

NM_003583

UniProt #

Q92630

Tag(s)

N-terminal His-tag

References

1. Taira,N. et al., Mol. Cell 25 (5), 725-738 (2007).
2. Wang,X. et al.,Mol. Cell. Biol. 23 (5), 1546- 1557 (2003).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing DYRKtide substrate peptide 1 (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Dual Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40053 Lot 220126-1

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