Cot (MAP3K8), GST-His-Tags Recombinant

Catalog #

40050

$355 *

Size: 10 µg

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Description

Recombinant human Cot, also known as MAP3K8 (mitogen-activated protein kinase kinase kinase 8), encompassing amino acids 30-397. This construct contains an N-terminal GST followed by a His-Tag (6xHis). The recombinant protein was affinity purified

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Synonyms

COT, TPL2, MEKK8, mitogen-activated protein kinase kinase kinase 8, Cancer Osaka Thyroid oncogene, Ewing Sarcoma Transformant, EST, Tumor Progression Locus 2, MAP3K8

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

COT Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

Cot (GST-His-30-397)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

72 kDa

Amino Acids

30–397

Specific Activity

47 pmol/min/µg

Genbank #

NM_005204

UniProt #

P41279

Tag(s)

N-terminal GST-His-tags

Background

COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-kappaB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.

References

1. Stafford, M.J. et al., FEBS Lett. 580, 4010-4014 (2006).
2. Babu, G. et al., J. Biol. Chem. 281, 14041-14047 (2006).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Activity was measured using Kinase Buffer 1 (BPS Bioscience #79334) with MEK1 substrate (0.2 mg/ml) and 20 µM ATP. Reaction was incubated at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40050 Lot 230127-G1

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Cot (MAP3K8), GST-His-Tags Recombinant

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