CERK, FLAG-Tag (Mouse) Recombinant

Catalog #

79413

$425 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Mouse CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system.

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Synonyms

ceramide kinase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥51%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide

61 kDa

Amino Acids

1-531 (end)

Specific Activity

≥10 pmole/min/μg

Genbank #

NM_00113486

Tag(s)

C-terminal FLAG-tag

References

1. Sugiura, M. et al., JBC. 277, 23294-23300 (2002)

2. Majjalieh, S.M., et al., JBC. 264, 14354-14360 (1989)

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay conditions: CERK activity was measured at 37^oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Kinase

Regulatory Status

Research Use Only