CERK (K298G, K303G), FLAG-Tag Recombinant
Recombinant human CERK (ceramide kinase), encompassing amino acids 1-537(end). This construct contains a C-terminal FLAG-tag and two mutations of interest (K298G and K303G). This protein was affinity purified.
≥85%
Aqueous buffer solution.
20 mM Tris-HCl, pH 7.4, 104 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and 80 µg/ml FLAG peptide
CERK (ceramide kinase), also known as acylsphingosine kinase, is a protein of the phosphotransferase family involved in ceramide phosphorylation in cell survival and proliferation and support of phagosome formation in leukocytes. It is found mostly in neutrophils, cerebrum granule cells and epithelial lung cells. The human gene includes a RARE (retinoic acid response element)-like transcription motif, which may function to promote expression of CERK in 5H-SY5Y cells when ATRA (all-trans retinoic acid) is present. The phosphorylation of ceramides produces C-1-P (ceramide-1-phosphate), which can help bring cPLA2 (cytosolic phospholipase A2) and CERK together and activate cPLA2. It was found that TPBC (triple-positive breast cancer) and TNBC (triple-negative breast cancer) present CERK dysfunction, and that inhibition of CERK expression or activity could slow down tumor growth. CERK may thus be a promising target in TNBC and TNPC, which currently have very limited therapeutic options.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.