CECR2, His-tag Recombinant

Catalog #

31046

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain CECR2 (cat eye syndrome chromosome region, candidate 2), GenBank Accession No. NM_031413, a.a. 430-543 corresponding to single bromodomain with N-terminal His-tag, MW = 14.4 kDa, expressed in an E. coli expression system.

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Synonyms

CECR2, cat eye syndrome chromosome region, candidate 2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.

14.4 kDa

Amino Acids

430-543

Genbank #

NM_031413

UniProt #

Q9BXF3

Tag(s)

N-terminal His-tag

References

1. Chen J, et al.,Int J Dev Biol. 2010;54(5):925-9.
2. 2. Lee SK, et al., Mol Cells. 2012 Jul;34(1):85-91.

Storage/Stability

>6 months at –80°C.

Assay Conditions

CeCR2 was incubated with various amounts of BET ligand in assay buffer in a 10 µl reaction for 30 min at RT. Reactions with H4 1-21[B] were prepared in parallel as control. AlphaLISA^® ® Ni acceptor beads (1:250) were added and incubation continued for 30 min more. Finally, Streptavidin Donor beads (1:125) were added and mixture was incubated for 10 min at RT before A-counts were measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only