Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Neuro2A cell pool can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
CRISPR cell pool, Neuro2A cell pool, Cas9 cell pool, mouse neuroblastoma, Adherent neuroblast cells, Nectin4, Nectin-4, Nectin
Neuro2a is a mouse neuroblastoma cell line. Adherent neuroblast cells.
Species
S. pyogenes
Supplied As
Each vial contains ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
UniProt #
Q99ZW2
Mycoplasma Testing
This cell pool has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Storage/Stability
Immediately upon receipt, store in liquid nitrogen.
Culture Conditions
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1X MEM Non-essential Amino Acids, 1% Sodium Pyruvate, and 1% Penicillin/Streptomycin
Growth Medium 1Q (BPS Bioscience #78096): MEM medium supplemented with 10% FBS, 1X MEM Non-essential Amino Acids, 1% Sodium Pyruvate, and 1% Penicillin/Streptomycin plus 1 μg/mL Puromycin to ensure recombinant expression.
Instructions for Use
See assay protocol for detailed instructions.
Shipping Temperature
-80°C
Notes
The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.
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