Cas9-Expressing Neuro2A Cell Line (High or Low Expression)
This cell line is a clonal derivative from the Cas9-Expressing Neuro2A Cell Pool (BPS Bioscience, #78087). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing (78137-H) or low-expressing (78137-L) cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
| Materials Required for Cell Line Culture | |
| Name | Ordering Information |
| Thaw Medium 1 | BPS Bioscience #60187 |
| Growth Medium 1Q | BPS Bioscience #78096 |
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Neuro2A cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
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