Cas9 (D10A, H840A), NLS, His-Tag (S. pyogenes) Recombinant

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Catalog #

101633

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Description

Recombinant S. pyogenes SpCas9(D10A, H840A)-NLS (conserved hypothetical protein), full length. This construct contains a C-terminal His-Tag. SpCas9(D10A, H840A)-NLS is a catalytic mutant of Cas9 that contains a nuclear localization sequence for optimal activity.

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4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Streptococcus pyogenes

Construct

Cas9 (D10A, H840A) (Full Length-NLS-His) (S. pyogenes)

Mutation

D10A, H840A

Host Species/Expression System

E. coli

Purity

80%

Format

Aqueous buffer solution.

Formulation

50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 1 mM DTT, 200 mM imidazole and 10% glycerol

160 kDa

Genbank #

AAK33936

Tag(s)

C-terminal His-tag

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Datasheets/Protocols

101633 lot 230110

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Cas9 (D10A, H840A), NLS, His-Tag (S. pyogenes) Recombinant

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