CARP2, SUMO-His-tags

Catalog #: 81050
Size: 25 µg
Species: Human
Host Species: E. coli
MW: 41 kDa
Genbank #: NP_476519
Tag(s): N-terminal SUMO-His-tags
a.a: full length
CARP2 (caspase 8/10 associated RING protein 2) is a RING-domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates, along with E1 and E2 enzymes. CARPs (CARP1 and CARP2, 77% identity) also belong to the IAP family (inhibitors of apoptosis proteins) inhibiting activation of DED (death effector domain) containing caspase proteins (8 and 10). In addition to targeting caspases 8 and 10, CARPs have been shown to target phosphorylated p53 for degradation in an Hdm2 independent manner. Furthermore, CARP2 has been shown to be a negative regulator of TNF induced NF-kappaB activation by targeting RIP for degradation.
Human CARP2 recombinant protein (Genbank Accession No. NP_476519), full length with N-terminal SUMO- and His-tags, expressed in E. coli.
RFFL, ring finger and FYVE-like domain containing 1
≥95% by SDS-PAGE
Biological Activity:
Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Aqueous buffer solution
Storage / Stability:

Stable for ≥1 year at –80°C.

Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
1. Yang, W., et al., CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation. J Biol Chem, 2007. 282:3273-81.
2. Yang, W. and W.S. El-Deiry, CARPs are E3 ligases that target apical caspases and p53. Cancer Biol Ther, 2007. 6:1676-83.
3. McDonald, E.R., 3rd and W.S. El-Deiry, Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. Proc Natl Acad Sci USA, 2004. 101:6170-5.
4. Liao, W., et al., CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Curr Biol, 2008. 18:641-9.
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (Ubiquitin Ligase (E3))
Product Type:
Recombinant Protein
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