CAMK2δ, GST-tag Recombinant

Catalog #

41109

$410 *

Size: 10 µg

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Bulk/Custom

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Description

Recombinant human calcium/calmodulin-dependent protein kinase 2 delta (CAMK2δ), also known as CAMKIIδ, CAMK2d and CAMKIId, GenBank Accession No. NM_172115, fulllength with an N-terminal GST tag and expressed in a baculovirus-infected Sf9 insect cell expression system. MW = 77 kDa.

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Synonyms

calcium/calmodulin-dependent protein kinase 2 delta, CAMKIIδ, CAMK2d, CAMKIId, CAMK-2

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 Insect Cells

Purity

≥85%

Format

Aqueous buffer solution.

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

77 kDa

Amino Acids

Full-Length

Specific Activity

5,860 pmol/min/µg

Genbank #

NM_172115

UniProt #

Q4G1A8

Tag(s)

N-terminal GST tag

References

1. Hoch, B., et al., Circ. Res. 1999; 84: 713- 721.
2. Zhu, W.-Z., et al., J. Clin. Invest. 2003; 111: 617-625.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Varying amounts of enzyme were incubated in 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 4 mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA, 0.4 mM EDTA, 0.01 mM DTT, 0.05 mM ATP, 7.5 μg of Autocamtide 2 synthetic peptide substrate, Ca2+/Calmodulin solution, and 32 μCi of [33P]-ATP was incubated at 30°C for 15 minutes. Then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

Dry ice

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase - Cytoplasmic

Regulatory Status

Research Use Only

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Datasheets/Protocols

41109_Lot141211.pdf

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