BRPF3 Inhibitor Screening Assay Kit

Catalog #: 32608
Size: 384 reactions
$995
Description:
The BRPF3 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRPF3 binding to its substrate. The key to the BRPF3 Inhibitor Screening Assay Kit is the specific binding of the BRPF3 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRPF3 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts
Synonym(s):
BRPF3, Bromodomain and PHD finger containing 3
Supplied As:
The kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged BRPF3 bromodomain to perform a total of 384 enzyme reactions.
Contraindications:
DMSO above 0.5%. Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Reference(s):
Filippakopoulos, P., et al., Cell 2012; 149:214.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

32608.pdf