BRPF3 TR-FRET Assay Kit

Catalog #: 32626
Size: 384 reactions
$995
Description:
The BRPF3 TR-FRET Assay Kit is designed to measure the inhibition of BRPF3 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRPF3, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Materials Required But Not Provided:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

UniProt
Q9ULD4
Synonym(s):
KIAA1286, HTRF
Supplied As:
Kit comes in a convenient format with purified BRPF3 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Format:
COMPONENTS
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Filippakopoulos, P., et al., Cell 2012; 149:214.
Notes:

The final concentration of DMSO should not exceed 0.5%.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

32626.pdf