The BRPF3 TR-FRET Assay Kit is designed to measure the inhibition of BRPF3 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRPF3, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
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Kit comes in a convenient format with purified BRPF3 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Materials Required But Not Supplied
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
Format
COMPONENTS
UniProt #
Q9ULD4
References
Filippakopoulos, P., et al., Cell 2012; 149:214.
Storage/Stability
At least 6 months from date of receipt when stored as directed.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Notes
The final concentration of DMSO should not exceed 0.5%.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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