SMARCA4/BRG1 Inhibitor Screening Assay Kit (Discontinued)
Catalog #
32609
$510
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Description
The BRG1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRG1 binding to its substrate. The key to the BRG1 Inhibitor Screening Assay Kit is the specific binding of the BRG1 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRG1 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
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Synonyms
BRG1, SMARCA4, activity assay kit
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
The kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged BRG1 bromodomain to perform a total of 384 enzyme reactions.
Materials Required But Not Supplied
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Format
COMPONENTS
UniProt #
Q9HBD4
References
Filippakopoulos, P., et al., Cell 2012; 149:214.