The SMARCA4 TR-FRET Assay Kit is designed to measure the inhibition of SMARCA4 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, SMARCA4, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against SMARCA4/BRG1? Check out our Bromodomain Screening Services.
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Synonyms
SMARCA4, BRG1, Actin Like 6A, ACTL6A, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1, SMARCB1, HTRF
*The concentrations of protein are lot-specific and will be indicated on the tubes containing the protein.
UniProt #
P51532
References
Schiaffino-Ortega, S., et al., 2014. J. Hematol. Oncol. 7:81.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Shipping Temperature
-80°C (dry ice)
Notes
The final concentration of DMSO should not exceed 0.5%.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.