BRAF, GST-tag Recombinant

Catalog #

40065

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human BRAF, also known as B-Raf Proto-Oncogene and RAFB1, GenBank Accession No. NM_004333, a.a. 417-766(end) with N-terminal GST-tag, expressed in Sf9 cells using a Baculovirus expression system. MW=65 kDa.

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Synonyms

B-RAF1, BRAF, RAFB1, B-Raf Proto-Oncogene

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥35%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% Glycerol.

65 kDa

Amino Acids

417–766 (end)

Specific Activity

≥112 pmole/min/μg

Genbank #

NM_004333

UniProt #

P15056

Tag(s)

N-terminal GST-tag

References

1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775–1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.

Storage/Stability

At least months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT, 0.1 mg/ml Raf substrate (Mek1 cat #40075 lot 190508-3), and 10 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40065 Lot 190418-G1.pdf

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BRAF, GST-tag Recombinant

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