Boc-D-FMK

Catalog #: 27611-1
Size: 5 mg
$210
MW: 263.3 Da
CAS Registry #: 187389-53-3,634911-80-1
Formula: C11H18FNO5
Target: pan-caspase
Background:
Boc-D-FMK is a cell-permeable broad-spectrum caspase inhibitor that fully inhibits the pro-apoptotic effect of tumor necrosis factor-α (TNFα) with IC50 value of 39 µM. Boc-D-FMK has been found to reduce the activation of nuclear factor kappa light chain enhancer of activated B cells (NF-kB), suppress the phosphorylation of subunit nuclear factor kappa light polypeptide gene enhancer in B cells inhibitor α (IkBα) and inhibits TNF-induced expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1). Moreover, Boc-D-FMK has also effectively attenuated the hepatocyte apoptosis in bile duct-ligated rats potentially improving the survival rates.
Description:
A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. Can be used for both in vitro and in vivo studies--see note for using this product in vitro.
Synonym(s):
Caspase Inhibitor III; Boc-Asp(OMe)-FMK; Boc-D(OMe)-FMK; Boc-Asp-Fluoromethylketone; methyl 5-fluoro-3-[(2-methylpropan-2-yl)oxycarbonylamino]-4-oxopentanoate
Purity:

≥98% by HPLC

Solubility:
Soluble in DMSO
Storage / Stability:

Store at or below –20°C. Solid form is stable at least 12 months from date of receipt, when stored as directed. Do not store aqueous solutions for more than one day.

Reference(s):
1. Cowburn AS,et al. Blood. 2005 Apr 1;105(7):2970-2.
2. Wu X, et al. Am J Physiol Renal Physiol. 2009 Aug;297(2):F316-26.
3. Sheen-Chen SM, et al. J Gastroenterol Hepatol. 2008 Aug;23(8 Pt 1):1276-9.
Notes:
The inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups.
Amino Acid Sequence:
Boc-Asp(OMe)-CH?F
Scientific Category:
Apoptosis Inhibitor
Product Type:
Inhibitor
Data shown is lot-specific. Contact us for specific information on other lots.

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