Rat bFGF Recombinant

Catalog #

90113-A

$130 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant Rat bFGF is a disulfide-linked homodimeric protein consisting of 146 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat basic Fibroblast Growth Factor mature chain was expressed in E. coli.

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Synonyms

bFGF, FGF2, Prostatropin, Basic Fibroblast Growth Factor, Fibroblast Growth Factor-2

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

E. coli

Purity

≥96% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS solution, pH 7.5.

16 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

10–154

Biological Activity

The ED₅₀ determined by dose-dependent stimulation of thymidine uptake by 3T3 cells expressing FGF receptors, was found to be <1 ng/ml.

Genbank #

P13109

UniProt #

P13109

Background

Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

References

1. Przybylski M. J Wound Care. 2009 Dec,18(12):516-9.
2. Temple S, Qian X. Neuron. 1995 Aug,15(2):249-52.

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Rat bFGF Recombinant

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