Ask1, GST-tag Recombinant

Catalog #

40028

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_005923.

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Synonyms

MAP3K5, MEKK5, MAPKKK5

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

60 kDa

Amino Acids

649–946

Specific Activity

96 pmol/min/µg

Genbank #

NM_005923

UniProt #

Q99683

Tag(s)

N-terminal GST-tag

Background

ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References

1. Ichijo, H. et al., Science 275: 90-94, 1997.
2. Nishitoh, H. et al., Molec. Cell 2: 389-395, 1998.

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Ask1, GST-tag Recombinant

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