Anti-V5-Tag, Monoclonal

Catalog #
25001
$275 *
Size: 100 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Affinity purified Monoclonal Anti-V5-Tag, clone E10/V4RR, mouse IgG1. Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. Affinity purified from culture supernatant of hybridoma cells. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA (1:5000-10000), Immunocytochemistry (1:500-2000), Immunoprecipitation , Frozen sections (1:500-2000), Paraffin sections. Optimal dilutions/concentrations should be determined by the end user.

Synonyms
Antibody, V5-tag
Product Info
Storage and Usage
Citations
Isotype
IgG1
Clonality
clone E10/V4RR
Host Species/Expression System
Mouse
Purification
Protein A Affinity Chromatography
Format
Aqueous buffer solution
Formulation
1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen
Synthetic peptide: GKPIPNPLLGLDST
Sensitivity
Recognizes 1-2 ng of V5-tagged fusion proteins.
Background
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 a.a. (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).