Anti–HP1α, ß and γ polyclonal antibody

Catalog #

25292

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human HP1β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the HP1α and HP1γ isoforms.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/25 µl

Immunogen

recombinant protein

Background

HP1 alpha, beta and gamma (UniProt/Swiss–Prot entry P45973, P83916 and Q13185) are components of heterochromatin. They recognize and bind histone H3 tails methylated at Lys–9, leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the inner nuclear membrane

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP results obtained with the antibody directed against HP1α, ß and γ
ChIP assays were performed using NIH3T3 cells and 4 µg of the antibody directed against HP1α, ß and γ (Cat. # 25292). QPCR was performed on the immunoprecipitated DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure 1 shows the relative enrichment as compared to a no antibody negative control ChIP.

Western blot analysis using the antibody directed against HP1α, ß and γ
Western blot was performed on nuclear extracts from HeLa cells (20 µg) with the antibody against human HP1α, ß and γ (Cat. # 25292) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right.

Immunofluorescence using the antibody directed against HP1α, ß and γ
HeLa cells were stained with the antibody against HP1α, ß and γ (Cat. # 25292) and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß, γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications

ChIP (4 µg/ChIP)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Scientific Category

Antibodies

Regulatory Status

Research Use Only