Anti-HP1α polyclonal antibody

Catalog #

25291

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human HP1α (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein

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Synonyms

CBX5, HP1A

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Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/25 µl

Immunogen

recombinant protein

Background

HP1 alpha (UniProt/Swiss–Prot entry P45973) is a component of heterochromatin. It recognizes and binds to histone H3 tails methylated at Lys–9, leading to epigenetic repression of transcription. HP1 alpha may also interact with lamin B receptor (LBR), thereby contributing to the association of the heterochromatin with the inner nuclear membrane. Further, HP1 alpha is involved in the formation of a functional kinetochore through interaction with MIS12 complex proteins.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the antibody directed against HP1α
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the antibody against human HP1α (Cat. No. 25291), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The Western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.

Immunofluorescence using the antibody directed against HP1α
HeLa cells were stained with the antibody against HP1α (Cat. No. 25291) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications

WB (1:1000)
IF: (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti-HP1α polyclonal antibody

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