ALK2 (ACVR1), GST-tag Recombinant

Catalog #

40019

$390 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ALK2 a.a. 147-509(end), Genbank Accession No. NM_001105.5, with an N-terminal GST-tag, expressed in Sf9 cells using a baculovirus expression system. MW = 67 kDa.

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Synonyms

ACVRLK2, FOP, SKR1, TSRI, ALK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20 % Glycerol and 3 mM DTT

67 kDa

Amino Acids

147-509 (end)

Specific Activity

40 pmol/min/µg

Genbank #

NM_001105.5

UniProt #

Q04771

Tag(s)

N-terminal GST-tag

Background

ALK 2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8. Knockdown of ALK2, but not TGFβRI (ALK5), abrogates endoglin-mediated decrease in cell motility of prostate cancer cells and constitutively active ALK2 is sufficient to restore a low-motility phenotype in endoglin deficient cells (1). Therefore, endoglin decreases prostate cancer cell motility through activation of the ALK2-Smad1 pathway. ALK2 is the key gene involved in Fibrodysplasia ossificans progressiva (FOP), a rare autosomal dominant congenital disorder characterized by progressive heterotopic bone formation in muscle tissues (2).

References

1. Craft, C.S. et al . Oncogene. 2007 Nov 8;26(51):7240-50.
2. Shore, E. M. et al . Nature Genet. 38: 525-527, 2006.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ALK2—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of BPS ADP-Glo reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of BPS Kinase Detection Reagent—incubated 45 minutes at room temperature. Luminescence was measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Serine-Threonine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40019 Lot 180816

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ALK2 (ACVR1), GST-tag Recombinant

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