p53 Luciferase Reporter Lentivirus

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Catalog #
78666
$880 *
Size: 500 µl x 2
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Description

The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the p53 luciferase reporter lentivirus.

Synonyms
cellular tumor antigen p53, tumor suppressor p53, TP53, p53
Product Info
Storage and Usage
Citations
Supplied As
Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Materials Required But Not Supplied
Name Ordering Information
HCT116 ATCC #CCL-247
Thaw Medium 7 BPS Bioscience #60185
Assay Medium 7A BPS Bioscience #78673
Mitomycin c Sigma #M4287-2MG
Nutlin-3  Sigma #N6287-1MG
Doxorubicin Sigma #D1515
96-well tissue culture, clear-bottom, white plate Corning #3610
One-Step Luciferase Assay System BPS Bioscience #60690
Luminometer  
Formulation

The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Background

p53 is a transcription factor and tumor suppressor very frequently mutated in human cancer and often termed “guardian of the genome”. Activated by DNA damage, oxidative stress, or deregulated oncogene expression, p53 binds to a specific site in the promoter region of target genes and leads to the transcriptional activation of downstream genes involved in DNA repair, cell cycle arrest, senescence, and apoptosis. Inactivation of p53 promotes genome instability and directly contributes to cell transformation.