Anti-PSMA Antibody

Catalog #

101695

$335 *

Size: 50 µg

Qty

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Description

Recombinant anti-PSMA monoclonal antibody which recognizes PSMA (prostate-specific membrane antigen). This antibody has been affinity purified.

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Synonyms

FOLH1, FOLH, NAALAD1, PSM, GIG27, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PSMA: Anti-PSMA Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

High Chain: 52 kDa; Low Chain: 23 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

Q04609

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

The antibody was validated by measuring binding to PSMA antigen in ELISA. The PSMA protein (BPS Bioscience #100463) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-PSMA antibody (BPS Bioscience #101695), serially diluted in Blocking Buffer 2, was added and incubated for 30 minutes at room temperature. After 3 more washes, the plate was incubated with anti-human IgG Fc HRP, washed, and incubated with HRP Colorimetric Substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying the binding to PSMA in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

101695 Lot 230214

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