Anti-PSMA, Biotin Label Antibody

Catalog #

101757

$450 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human anti-PSMA antibody recognizing human PSMA protein. This anti-PSMA antibody is a purified recombinant antibody, which contains an Avi-Tag™ and is biotinylated at the C-terminus of the heavy chain unit.

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Synonyms

FOLH1, Glutamate carboxypeptidase 2, folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, PSM, FGCP, FOLH, GCP2, PSMA, mGCP, GCPII, NAALAD1, NAALAdase, N-acetylated-alpha-linked acidic dipeptidase I

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PSMA: anti-PSMA-Biotin Binding Assay

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 52 kDa; Light Chain: 23 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

Q04609

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

PSMA (prostate-specific membrane antigen, also known as Folate hydrolase 1, FOLH1), is highly expressed in prostate cells and is used as a diagnostic and prognostic indicator of prostate cancer. The enzyme has folate hydrolase and peptidase activity. It plays a role in prostate cancer progression through the PI3K-Akt and MAPK-ERK1/2 pathways. PSMA/FOLH1 is used in the clinic in PET (positron emission tomography) imaging of prostate cancer whereas radioactive analogs are used for the treatment of prostate cancer. PSMA is also expressed in other tumor types and in a few normal tissues, such as kidney. PSMA/FOLH1 is a target of CAR-T cells and bi-specific antibodies are currently under development for cancer therapy.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The antibody was validated by measuring Anti-PSMA binding to PSMA antigen in ELISA assay. The PSMA protein (BPS Bioscience #100463) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-PSMA antibody (BPS Bioscience #101757), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. The plate was washed, incubated with Streptavidin-HRP, washed again, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying the binding of PSMA in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

101757 Lot 230428

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Anti-PSMA, Biotin Label Antibody

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