VPS34, FLAG-tag

Catalog #: 40690
Size: 20 µg
$385
Species: Human
Host Species: Sf9 insect cells
MW: 102.5 kDa
Genbank #: NM_002647
Tag(s): N-terminal FLAG-tag
a.a: 2–887 (end)
Description:
Human VPS34 (vacuolar protein sorting 34), GenBank Accession No. NM_002647, a.a. 2-888(end) with N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=102.5 kDa.
UniProt
Q8NEB9 P28221
Synonym(s):
VPS34, vacuolar protein sorting 34
Specific Activity:
100 pmol/min/μg.
Assay Conditions:
Assay was done in a Kinase buffer containing 2 mM McCl2 at 30°C for 20 min. Use BPS PI:4PS substrate (100 µM) with 100 µM ATP. The amount of ATP transferred was calculated using ADP-Glo reagent.
Formulation:
40mM Tris pH8.0, 110mM NaCl, 2.2mM KCl, 80µg/mL FLAG peptide, 20% glycerol, 3mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Yan, Y., et al. (2009). Biochem J. 417(3):747-55.
2. Backer, J.M. (2008). Biochem J. 410(1):1-17.
3. Juhász, G., et al. (2008). J Cell Biol. 181(4):655-66.

Application Reference(s):
1. Alteration of autophagy-related proteins in peripheral blood mononuclear cells of patients with Parkinson's disease (2017)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Lipids
Product Type:
Recombinant Protein
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Product Datasheets

40690_Lot_180611-1.pdf