USP5 (IsoT), FLAG-tag Recombinant

Catalog #

80355

$425 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (IsoT), GenBank Accession No. NM_001098536, a.a. 2-858(end) with an N-terminal FLAG-tag, MW=96 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

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Synonyms

USP 5, Ubiquitin-Specific Protease 5, Isopeptidase T, IsoT, USP-5

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

USP5 inhibition curve using a fluorogenic assay (#78832). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥51%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, and 90 ?g/ml FLAG peptide

96 kDa

Amino Acids

2–858(end)

Specific Activity

14.3 pmole/min/µg

Genbank #

NM_001098536

UniProt #

P45974

Tag(s)

N-terminal FLAG-tag

References

1. Dayal, S., et al. (2009). J. Biol. Chem. 284(8):5030-41.
2. Reyes-Turcu, F.E., et al. (2008). J. Biol. Chem. 283(28):19581-92.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP5 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80355_Lot120213.pdf

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USP5 (IsoT), FLAG-tag Recombinant

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