USP20, His-Tag, FLAG-Tag Recombinant

Catalog #

100178

$425 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human USP20, also known as ubiquitin specific peptidase 20 and Deubiquitinating Enzyme 20, GenBank Accession No. NM_006676, a.a. 2–915 (end) with N-terminal His-Tag and C-terminal FLAG-Tag, expressed in a HEK293 expression system. MW = 104 kDa.

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Synonyms

Ubiquitin specific peptidase 20, Deubiquitinating Enzyme 20, USP-20

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

USP20 Activity

USP20 inhibition curve using a fluorogenic assay (#78840). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

USP20 (His-2-915(end)-FLAG)

Host Species/Expression System

HEK293

Purity

≥85%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 µg/ml FLAG peptide, and 20% glycerol.

104 kDa

Amino Acids

2-915 (end)

Specific Activity

10 pmol/min/μg

Genbank #

NM_006676

UniProt #

Q9Y2K6

Tag(s)

N-terminal His-tag, C-terminal FLAG-tag

References

1. Berthouze, M., et al., EMBO J. 2009 June; 28(12):1684 - 1696.
2. Yasunaga, J., et al., J. of Virology.. Jun 2011, 85(13):6212-6219.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay performed according to fluorogenic USP20 Assay kit (BPS Bioscience #78840) with various amounts of USP20.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Ubiquitin

Regulatory Status

Research Use Only

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Datasheets/Protocols

100178 Lot 230310-1

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USP20, His-Tag, FLAG-Tag Recombinant

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