TYRO3 Kinase Assay Kit

Catalog #

79593

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

TYRO3 is a member of the TAM receptor kinases and plays an important role in cell proliferation/survival, adhesion/migration, and regulation of inflammatory cytokine release. Along with other members of this family, Axl and c-Mer (MERTK), TYRO3 is part of an emerging class of innate immune checkpoints participating in anti-tumoral immunity. Thus, it has been suggested that TYRO3 inhibition could be a promising immunotherapeutic approach in cancer treatment. The TYRO3 Assay Kit is designed to measure TYRO3 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against TYRO3? Check out our Kinase Screening Services.

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Synonyms

TYRO3, TYRO-3, BYK, RSE, Dtk, Sky, Tif, Brt

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The TYRO3 Assay Kit comes in a convenient 96-well format, with enough purified recombinant TYRO3 enzyme, TYRO3 substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

• Kinase-Glo MAX (Promega #V6071)
• Dithiothreitol (DTT, 1 M; optional)
• Microplate reader capable of reading luminescence
• Adjustable micropipettor and sterile tips
• 30°C incubator

Format

Assay Kit Components

UniProt #

Q06418

Background

TAM receptor protein tyrosine kinase TYRO3 is a ubiquitylation substrate for CBL-B and essential regulator of immune homeostasis. Deregulation of TAM signaling has been linked to the pathogenesis of autoimmune, inflammatory, and infectious diseases. TAM receptors have also been associated with cancer development and progression. In a cancer setting, TYRO3 has a dual regulatory role, controlling the initiation and progression of tumor development as well as the associated anti-tumor responses of diverse immune cells. Thus, modulation of TYRO3 has emerged as a potential novel strategy for cancer treatment.

TYRO3 inhibition or degradation plays a significant role in NK cell activation. Treatment of NK cells with a small molecule, a TAM kinase inhibitor, conferred therapeutic potential by efficiently enhancing anti-metastatic NK cell activity in vivo. The TAM inhibitor markedly reduced NK-dependent mammary cancer and melanoma metastases. TAM/CBL-B inhibitory pathway shows a possibility to develop new therapies that awakens the innate immune system to kill cancer metastases.

References

Akalu, Y.T., et. al. Immunological Reviews 276:165-177 (2017)

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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TYRO3 Kinase Assay Kit

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