Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway

Catalog #: 79265
Size: 500 reactions
$695
Background:

The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor can regulate the transcription of a large number of genes, including those that regulate glucose metabolism and inflammatory responses.

Description:

The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control.  It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells.

The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.

The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.

Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep.  Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity.  The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

*Note:  the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Synonym(s):
GAL4, Glucocorticoid Receptor, Galectin
Concentration:
Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
Format:

Instructions for use:
See data sheet insert for detailed transfection and assay protocols.
Storage / Stability:
Stable at least 12 months from date of receipt, when stored as directed (-20°C)
Application(s):
Monitor glucocorticoid signaling pathway activity and screen activators or inhibitors of the glucocorticoid signaling pathway.
Notes:
Note: These vectors are ready-to-use for transient transfection. They are NOT SUITABLE for transformation and amplification in bacteria.
Warning(s):
Avoid freeze/thaw cycles
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79265.pdf