TNKS1 (PARP5A) Colorimetric Assay Kit

Catalog #

78576

$875 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TNKS1 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 1 (TNKS1, also known as PARP5A) for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The TNKS1 assay kit comes in a convenient 96-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions. The key to the TNKS1 (PARP5A) Colorimetric Assay Kit is the biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.

Figure 1. TNKS1 (PARP5A) Colorimetric Assay Kit schematic

*NOTE: As of March 2023, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request

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Synonyms

Tankyrase I, Tankyrase-1, TANK1, TNKS, PARPL, TIN1, TINF1, Poly [ADP-ribose] polymerase tankyrase-1, ADP-ribosyltransferase diphtheria toxin-like 5, ARTD5, Poly [ADP-ribose] polymerase 5A, Protein poly-ADP-ribosyltransferase tankyrase-1, TNKS-1

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Product Data Gallery

TNKS1 (PARP5A) activity in the presence of increasing concentrations of XAV939

Product Info

Storage and Usage

Citations

Assay Kit Format

Colorimetric

Materials Required But Not Supplied

DTT (10 mM in water, prepared fresh)
1x PBS (phosphate buffer saline)
PBST: 1x PBS, containing 0.05% Tween-20
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
2 M sulfuric acid (aqueous)
Adjustable micropipettor and sterile tips
Rotating or rocker platform

*Alternately, a spectrophotometer reading at 650 nm may be used, but sensitivity of the assay will be greatly reduced.

Format

Catalog #	Name	Amount	Storage
80504	TNKS1* (Tankyrase 1 (PARP5A), GST-Tag)	3 µg	-80°C	Avoid multiple freeze/ thaw cycles
52029	5x Histone Mixture	1 ml	-80°C
78371	PARP Substrate Mixture 2	4 x 250 µl	-80°C
80602	10x PARP Assay Buffer	1 ml	-20°C
79743	Blocking Buffer 3	25 ml	+4°C
80611	Streptavidin-HRP	100 µl	+4°C
79651	Colorimetric HRP Substrate	10 ml	+4°C
79964	96-well clear plate		Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube. Excess material has been given for ease of retrieval.

UniProt #

O95271

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The TNKS1 (PARP5A) Colorimetric Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution and using 5 µl per well.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

PARP

Regulatory Status

Research Use Only