TNKS1 Homogenous Assay Kit

Catalog #

78489

$1,470 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TNKS1 (also known as PARP5a) Homogeneous Assay Kit is designed to measure TNKS1 activity for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS1 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, a sample containing TNKS1 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

TNKS1 Homogenous Assay Kit

Figure 1: TNKS1 (PARP5a) Homogenous Assay Kit schematic

Need us to run inhibitor screens or profile your compounds against TNKS1? Check out our PARP/PARPTrap™ Screening Services or DNA Replication and Repair Services.

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Synonyms

PARP5A, Poly [ADP-ribose] polymerase tankyrase-1, ADP-ribosyltransferase diphtheria toxin-like 5, ARTD5, Poly [ADP-ribose] polymerase 5A, Protein poly-ADP-ribosyltransferase tankyrase-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, TANK1, TNKS-

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

The TNKS1 Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with biotinylated histone substrate, recombinant antibody (ADP-ribose binding reagent 1), PP-01 assay buffer, and purified TNKS1 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA anti-rabbit IgG acceptor beads (Perkin Elmer #AL104C)
AlphaScreen Streptavidin-conjugated donor beads (Perkin Elmer #6760002S)
Optiplate -384 (Perkin Elmer #6007290)
AlphaScreen microplate reader
0.5 M DTT

Format

Catalog #	Name	Amount	Storage
80504	TNKS1 (PARP5a)*	2 µg	-80°C	Avoid multiple freeze/ thaw cycles
	5X PP-01 assay buffer	2 x 1 ml	-80°C
	Biotinylated histone substrate**	500 rxns	-80°C
78311	ADP-ribose binding reagent 1	10 µl	-80°C
	NAD+ (750 µM)	400 µl	-80°C
52301	4X Detection buffer 1	2 ml	-80°C

*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.
**Reconstitute in 500 µl H₂O.

UniProt #

O95271

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Screen for molecules that inhibit PARP1 activity.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The TNKS1 Homogenous Assay Kit is compatible with up to 1% final DMSO concentration.

We recommend preparing the inhibitor in no higher than 5% DMSO solution in buffer and using 3 µl per well.

Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

PARP

Regulatory Status

Research Use Only

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TNKS1 Homogenous Assay Kit

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