Human Tumor Necrosis Factor-beta Recombinant

Catalog #

90245-A

$130 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Recombinant TNF-beta is a non disulfide-linked homotrimer protein, each consisting of 172 amino acids and migrates as an approximately 21 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Tumor Necrosis Factor-beta chain was expressed in E. coli.

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Synonyms

TNF-beta TNFb, Tumor Necrosis Factor-beta, Lymphotoxin Alpha, LT-alpha

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.5.

21 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

35–205

Biological Activity

The ED₅₀ was measured in a cytotoxicity assay using mouse L929 cells in the presence of the metabolic inhibitor actinomycin, and was determined to be less than 0.1 ng/ml.

Genbank #

P01374

UniProt #

P01375

Background

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-α, TNF-β is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF- β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells.

References

1. Ishibashi, K., et al. Leuk Lymphoma. 1992 Aug,7(5-6):409-17.
2. Tracey KJ, Cerami A. Annu Rev Med. 1994,45:491-503
3. Sedgwick JD, et al. Immunol Today. 2000 Mar,21(3):110-3.

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Human Tumor Necrosis Factor-beta Recombinant

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