TBK1, GST-Tag Recombinant

Catalog #

40286

$375 *

Size: 10 µg

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Description

Recombinant human TBK1 (TANK-binding kinase 1), also known as NF-kappa-B-activating kinase, full length encompassing amino acids 2-279. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage Site for the removal of the tag. The recombinant protein was affinity purified.

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Synonyms

NAK, T2K, FLJ11330, TBK,Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, FTDALS4, NAK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT

110 kDa

Amino Acids

2-729(end)

Specific Activity

70 pmol/min/µg

Genbank #

NM_013254

UniProt #

Q9UHD2

Tag(s)

N-terminal GST-tag

Background

TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases (1). Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon (2).

References

1. Tojima, Y. et al: NAK is an I-kappa-B kinase-activating kinase. Nature 404: 778-782, 2000.
2. Sharma, S. et al: Triggering the interferon antiviral response through an IKK-related pathway. Science 300: 1148-1151, 2003.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

TBK1 activity was confirmed using the TBK1 Assay Kit (BPS Bioscience #79871) with TBK1 titrated from 400 ng/rxn 6.25 ng/rxn and 20 µM ATP used instead of 10 µM ATP as instructed in the kit.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40286 Lot 210122-G

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TBK1, GST-Tag Recombinant

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