SUV4-20H1, GST-tag Recombinant

Catalog #

51090

$440 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human SUV4-20H1 (GenBank Accession No. NM_017635), a.a. 2-387, with N-terminal GST-tag, MW= 70 kDa, expressed in an E. coli expression system.

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Synonyms

KMT5B

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥31%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and variable glutathione

70 kDa

Amino Acids

2–387

Genbank #

NM_017635

UniProt #

Q4FZB7

Tag(s)

N-terminal GST-tag

References

1. Yang H. et al., 2008 J Biol Chem. 2008 May 2;283(18):12085-92.
2. Benetti R et al., J Cell Biol. 2007 Sep 10;178(6):925-36.

Storage/Stability

>6 months at –80°C.

Assay Conditions

50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 800 µM S-adenosyl methionine, and SUV4-20H1) is added to the wells coated with the substrate. Incubate for 4 hr. Add primary antibody 18, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only