STK33, GST-tag Recombinant

Catalog #

40093

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

STK33 (GenBank Accession No. NM_030906.2), a.a. 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

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4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥46%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 115 mM NaCl, 10% glycerol, and 2 mM DTT.

84 kDa

Amino Acids

2–end

Specific Activity

≥10 pmol/min/µg

Genbank #

NM_030906.2

UniProt #

Q9BYT3

Tag(s)

N-terminal GST-tag

Background

STK33 is a distant member of the CAMK group of serine/threonine kinases but it lacks the calcium/calmodulin-binding domain and the C-terminal regulatory tail. STK33 is highly expressed in testis, lung epithelia, alveolar macrophages, horizontal cells in the retina and in embryonic organs such as heart, brain and spinal cord. STK33 is essential for abnormal cell growth in human cell lines expressing oncogenic mutations in KRAS, but not in human cancer cell lines expressing wildtype KRAS. STK33 is required for survival and proliferation of mutant KRAS-dependent cancer cells, in which it suppresses the S6K1-BAD proapoptotic signaling pathway.

References

1. Mujica, A.O., et al., 2001. Gene 280:175-181.
2. Brauksiepe, B., et al., 2008. Biochemistry 9:25-37.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was done using ADP-Glo (Promega) reagents in Kinase buffer containing 2 mM DTT using MBP substrate (0.1 mg/ml) and 100 μM ATP. Reaction was done at 30°C for 40 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40093_Lot_170929-G2.pdf

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