SOS1, FLAG-Tag Recombinant

Catalog #

101573

$335 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human SOS1 (son of sevenless homolog 1), encompassing amino acids 564-1049. This construct contains a C-terminus Flag-tag. This recombinant protein was affinity purified.

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Synonyms

Son of sevenless homolog 1, SOS-1, GF1, HGF, NS4, GGF1, GINGF

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

KRAS (G12C) Activation by SOS1 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

SOS1 (564-1049-FLAG)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

58 kDa

Amino Acids

564-1049

Genbank #

NM_005633

UniProt #

Q07889

Tag(s)

C-terminal FLAG Tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay quantifies SOS1-mediated loading of KRAS (G12C)–GDP with a fluorescent GTP analogue. Detection of successful loading was achieved by measuring resonance energy transfer from anti-His-terbium (FRET donor) bound to His-KRAS (G12C) to the loaded fluorescent GTP analogue (FRET acceptor). All steps of the assay were performed at room temperature. A volume of 5 µl of the KRAS (G12C) working solution and 5 µl of anti-His-terbium was added to all wells of the test plate. After 10 minutes, 5 µl of the SOS1 working solution and 5 µl 200 nM EDA–GTP–DY-647P1 was added to the wells. After 30 minutes incubation, HTRF was measured. Increase in TR-FRET is proportional to the exchange.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ras Signaling

Regulatory Status

Research Use Only

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Datasheets/Protocols

101573 Lot 230524-1

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SOS1, FLAG-Tag Recombinant

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