SOS1, FLAG-Tag, Avi-Tag, Biotin-Labeled Recombinant

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Catalog #

100753

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Description

Human Son of sevenless homolog 1 (SOS1), also known as guanine nucleotide exchange factor 1 (GEF1), encompassing amino acids 594-1049. This construct contains an N-terminal FLAG-tag and a C-terminal Avi-tag™. The recombinant protein was affinity purified and enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

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Synonyms

SOS-1, GF1, GGF1, GINGF, HGF, NS4, SOS Ras/Rac guanine nucleotide exchange factor 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Human

Construct

SOS1 (FLAG-594-1049-Avi)-(Biotin)

Host Species/Expression System

Sf9

Purity

≥85%

Supplied As

Aqueous buffer solution.

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and 80 µg/ml FLAG peptide

57 kDa

Amino Acids

594-1049

Genbank #

NM_005633

UniProt #

Q07889

Tag(s)

N-terminal FLAG-Tag, C-terminal Avi-Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Shi, H., et al., Plant Cell. 2002; 14: 465-477.
2. Shi, H., et al., PNAS. 2000; 97(12): 6896-6901.

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Datasheets/Protocols

100753 Lot 200603

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SOS1, FLAG-Tag, Avi-Tag, Biotin-Labeled Recombinant

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