KRAS (G12C), Isoform A, His-Tag, GDP-Loaded Recombinant

Catalog #

100640

$1,075 *

Size: 4 x 50 µg

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Description

Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease #50308). The protein also contains cancer-associated mutation G12C. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column. Ready for use in the GDP/GTP exchange assay or as a negative control for KRAS-RAF interaction.

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Synonyms

C-K-RAS, CFC2, K-ras, kRas(G12C), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

RBD-RAF1 (2.5 nM)/KRAS (G12C)

Bio-layer Interferometry (GATOR)

Product Info

Storage and Usage

Citations

Species

Human

Construct

KRAS (G12C) (His-TEV-2-186(end)) - (GDP)

Mutation

G12C

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT.

23 kDa

Amino Acids

2-186(end)

Genbank #

NM_033360

UniProt #

P01116

Tag(s)

N-terminal His-tag

References

1. Ostrem, J. M., et al., Nature. 2013;503:548-551.
2. Patricelli, M. P., et al., Cancer Discovery. 2016;6:316-329.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

KRAS (G12C) GDP-loaded was tested for binding to the RBD of Raf1. KRAS (G12C) GDP-loaded was compared with KRAS (G12C) GppNHp-loaded (BPS Bioscience #100641) for RBD-RAF1 (BPS Bioscience #100519) binding by Bio-layer Interferometry (GATOR, Probe Life) and AlphaScreening (PerkinElmer).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

100640 Lot 200207-D

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KRAS (G12C), Isoform A, His-Tag, GDP-Loaded Recombinant

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