KRAS (G12C), Isoform A, His-Tag (E. coli-derived) Recombinant

Catalog #

100413

$335 *

Size: 100 µg

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Description

Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308) and mutation of interest (G12C). The recombinant protein was affinity purified.

This product has been cited 1 time.

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Synonyms

C-K-RAS, CFC2, K-ras, kRas(G12C), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations1

Species

Human

Construct

KRAS (G12C) (His-TEV-2-186(end))

Mutation

G12C

Host Species/Expression System

E. coli

Purity

88%

Format

Aqueous buffer solution.

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT

23 kDa

Amino Acids

2-186(end)

Genbank #

NM_033360

UniProt #

P01116

Tag(s)

N-terminal His-Tag

References

1. Lito, P., et al., Science. 2016 Feb;351(6273):604-608.
2. Dogan, S., et al., Clinical Cancer Research. 2012 Nov;18(22):6169-6177.

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Datasheets/Protocols

100413 Lot 210921-B

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KRAS (G12C), Isoform A, His-Tag (E. coli-derived) Recombinant

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