KRAS (G13D), Isoform B, His-Tag Recombinant

Catalog #

100479

$320 *

Size: 100 µg

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Description

Recombinant human KRAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The protein also contains mutation of interest G13D. The protein was affinity purified.

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Synonyms

C-K-RAS, CFC2, K-ras, kRas(G13D), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2

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Product Data Gallery

6-20% SDS-Page Coomassie Staining

Intrinsic GTPase Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

KRAS (G13D) (His-2-185)

Mutation

G13D

Host Species/Expression System

Sf9

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 180 mM Imidazole

22 kDa

Amino Acids

2-185

Genbank #

NM_004985

UniProt #

P01116

Tag(s)

N-terminal His-Tag

References

1. Roock, W., et al., JAMA. 2010: 304(16): 1812-1820..
2. Kumar, S., et al., Gastrointestinal Cancer Research. 2014. Jan;7(1): 23-26.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Intrinsic GTPase reaction of KRAS (G13D) was conducted at room temperature for 60 min using GTPase-Glo (Promega) reagents with 1 µM GTP.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

100479 Lot 150811-1

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KRAS (G13D), Isoform B, His-Tag Recombinant

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