KRAS (G12D), Isoform A, His-Tag Recombinant
Catalog #
100623
$335
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Description
Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified.
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Synonyms
C-K-RAS, CFC2, K-ras, kRas(G12D), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2
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Product Data Gallery
Product Info
Storage and Usage
Citations
Species
Human
Construct
KRAS (G12D) (His-TEV-2-186(end))
Mutation
G12D
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution.
Formulation
20 mM HEPES, pH 7.4, 150 mM NaCl, 10% Glycerol, and 1 mM DTT
MW
23 kDa
Amino Acids
2-186
Genbank #
NM_033360
UniProt #
P01116
References
1. Lito, P., et al., Science. 2016 Feb;351(6273):604-608.
2. Dogan, S., et al., Clinical Cancer Research. 2012 Nov;18(22):6169-6177.