KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant

Catalog #

101312

$1,075 *

Size: 4 x 50 µg

Qty

Bulk/Custom

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Description

Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The recombinant protein also contains a mutation of interest, G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.

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Synonyms

C-K-RAS, CFC2, K-ras, kRas(G12D), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS2, RASK2, KI-RAS, K-RAS2A, c-Ki-ras, c-Ki-ras2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

KRAS (G12D) GDP-Loaded Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

KRAS (G12D) (His-TEV-2-186)-(GDP)

Mutation

G12D

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT

23 kDa

Amino Acids

2-186

Genbank #

NM_033360

UniProt #

P01116

Tag(s)

N-terminal His-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) with GDP loaded KRAS (G12C) (BPS Bioscience #100640) and GDP loaded KRAS (G12D) (BPS Bioscience #101312) titrated from 200 nM to 0 nM. Enzyme was incubated with SOS1; positive results show that SOS1 mediated exchange and subsequent RAS-Raf binding is successful.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

101312 Lot 211203

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KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant

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