KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant

Catalog #
101481
$995 *
Size: 4 x 60 µg
Qty
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Description

Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.

Synonyms
GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS2, RASK2
Product Info
Storage and Usage
Citations
Species
Human
Construct
KRAS (G12D) (His-TEV-2-186(end))-(GppNHp)
Mutation
G12D
Host Species/Expression System
E. coli
Purity

≥90%

Format

Aqueous buffer solution 

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT 

MW
23 kDa
Amino Acids
2-186(end)
Genbank #
NM_033360
UniProt #
P01116
Tag(s)
N-terminal His-tag