SMURF1, FLAG-tag Recombinant

Catalog #

80402

$390 *

Size: 20 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human SMURF1 (GenBank Accession No. NM_020429), a.a. 150-end, with N-terminal FLAG-tag, MW= 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

●

Synonyms

SMAD specific E3 ubiquitin protein ligase 1, SMURF1

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

SMURF1 inhibition curve using a TR-FRET assay (#78303). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

70 kDa

Amino Acids

150–end

Genbank #

NM_020429

UniProt #

Q9HCE7

Tag(s)

N-terminal FLAG-tag

References

1. Narimatsu M. et al., Cell 137 (2), 295-307 (2009)
2. Fukunaga E. et al., J. Biol. Chem. 283 (51), 35660-35667 (2008).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Assay Conditions

10 uL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 1 mM DTT, 0.2 µM Ub[Biotin], 50 nM Ube1, 1 mM ATP, 0.25 µM of UBCH5b, and various amount of smurf1. The reaction was initiated with the addition of ATP, and incubated at 30C for 3 hours. Tb labeled anti-Flag antibody donor and xl665 labeled acceptor was added and incubated at room temperature for hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

80402_Lot120103.pdf 80402_Lot_160314-G2.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

SMURF1, FLAG-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY