Sirtuin 6, GST-tag

Catalog #: 50017
Size: 100 µg
$425
Species: Human
Host Species: E. coli
MW: 65 kDa
Genbank #: NM_016539
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human Sirtuin 6 (GenBank Accession No. NM_016539), full length with N-terminal GST-tag, MW= 65 kDa, expressed in an E. coli expression system.
UniProt
Q8N6T7
Synonym(s):
Sirtuin6, SIRT6
Specific Activity:
1.5 pmol/min/µg
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS SIRT6 substrate 2 (Catalog #50084), 500 µM NAD+ and Sirtuin 6 (18 – 2000 ng). Incubation condition: 60 min at 37ºC followed by developing for 15 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Kanfi, Y. et al., FEBS Lett. 2008 Mar 5;582(5):543-8.
2. Mostoslavsky, R. et al., Cell. 2006 Jan 27;124(2):315-29.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50017_Lot_170928-1.pdf