ROS1 (G2032R), GST-Tag Recombinant

Catalog #

101640

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ROS1 (G2032R) (ROS proto-oncogene 1, receptor tyrosine kinase), encompassing amino acids 1881-end. This construct contains an N-terminal GST-tag and was affinity purified.

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Synonyms

Proto-oncogene tyrosine-protein kinase ROS, MCF3, ROS

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

ROS1 (G2032R) (GST-1881-end)

Mutation

G2032R

Host Species/Expression System

Sf9

Purity

90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

82 kDa

Amino Acids

1881-end

Specific Activity

141 pmol/min/µg

Genbank #

NM_002944

UniProt #

P08922

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

ROS1 activity was measured by using a IGF1Rtide synthetic peptide (KKKSPGEYVNIEFG), diluted in 20 mM Tris-HCl, pH 7.5 to a final concentration of 1 mg/ml, in the ADP Glo™ assay (Promega #V9101). Reaction was initiated by mixing increasing amounts of the ROS1 with 25 µM ATP in 40 mM Tris-HCl, pH 7.4, 25 mM MgCl₂, 0.1 mg/ml BSA prepared with 50 µM DTT, and substrate at a final concentration of 200 µg/ml final concentration.

After a 40-minute incubation at Room Temperature, the reaction was terminated by addition of ADP-Glo™ Reagent, followed by a subsequent 40-minute incubation at room temperature. Kinase Detection Reagent was added, and the reaction was incubated for another 30 minutes at ambient temperature. Detection of luminescence was measured using the Luminescence Module Protocol on GloMax®-Multi Microplate Multimode Reader. The corrected activity (RLU) was calculated by removing the blank value for each sample. The Kinase Specific Activity was calculated as follows: RLU / [(specific activity of ADP in RLU/pmol)*(Reaction time in min)*(Enzyme amount in µg or mg)]. The blank was determined from a “no kinase” sample by replacing the enzyme solution with an equal volume of Kinase Dilution Buffer IX (1X).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only