PRMT5/MEP50, FLAG-Tag, His-Tag (HEK293-derived) Recombinant

Catalog #

51045

$595 *

Size: 20 µg

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Description

Complex of human recombinant PRMT and MEP50 proteins. Recombinant human PRMT5 (protein arginine methyltransferase 5, variant 1), encompassing amino acids 2-637(end). This construct contains an N-terminal Flag-tag. Recombinant human MEP50 (WD repeat domain 77, variant 2), encompassing amino acids 2-342(end). This construct contains an N-terminal His-tag (6xHis). The two proteins were co-expressed and affinity purified.

This product has been cited 11 times.

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Synonyms

PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT Protein, PRMT-5

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PRMT5/MEP50 Activity

PRMT5 inhibition curve using a chemiluminescent assay (#52002L). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations11

Species

Human

Construct

PRMT5 (FLAG-2-637(end)) / MEP50 (His-2-342(end))

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and 100 µg/ml FLAG peptide

PRMT5: 74 kDa; MEP50: 38 kDa

Amino Acids

PRMT5: 2-637(end); MEP50: 2-342(end)

Genbank #

PRMT5: NM_006109; MEP50: NM_024102

UniProt #

O14744

Tag(s)

N-terminal FLAG-tag (PRMT5), N-terminal His-tag (MEP50)

Background

Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the PRMT5 Chemiluminescent Assay Kit (BPS Bioscience #52002L) with PRMT5 enzyme titrated from 800 ng/rxn – 0.78 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

51045 Lot 210405

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PRMT5/MEP50, FLAG-Tag, His-Tag (HEK293-derived) Recombinant

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