PRMT4 Chemiluminescent Assay Kit

Catalog #

52041L

$750 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

This product has been cited 4 times.

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Synonyms

PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1, PRMT-4

Product Info

Storage and Usage

Citations4

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with 8-well strips precoated with the specific PRMT4 peptide substrate, reagents, and purified PRMT4 enzyme for 96 enzyme reactions.

Format

Catalog #	Component	Amount	Storage
51047	PRMT4/CARM1 enzyme	20 µg	-80°C	*(Avoid freeze/ thaw cycles!)*
52120	20 µM S-adenosylmethionine	250 µl	-80°C
52140I	Primary antibody 9	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
	1% BSA	50 µl	-80°C
52191	4x HMT assay buffer 5*	3 ml	-20°C
52100	Blocking Buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate (2 components)	6 ml each	+4°C
	96-well plate precoated with PRMT4 substrate	1 plate	+4°C

UniProt #

Q86X55

Background

PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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PRMT4 Chemiluminescent Assay Kit

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