PLA2G4A, FLAG-His-tags Recombinant

Catalog #

60094

$375 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human PLA2G4A, GenBank Accession No. NM_024420, a.a. 1-749 (end), with C-terminal FLAG-His-tag, MW=87 kDa, expressed in a baculovirus expression system.

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Synonyms

Phospholipase A2 G4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 cells

Purity

≥87%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.

87 kDa

Amino Acids

1-749 (end)

Specific Activity

≥57 pmol/min/µg.

Genbank #

NM_024420

UniProt #

P47712

Tag(s)

C-terminal FLAG-His-tags

References

1. Chalimoniuk M. Postepy Biochem. 2012; 58(2):204-8.
2. Nadalin S, Giacometti J, et al., Prostaglandins Leukot Essent Fatty Acids. 2013; 88(2):185-90.

Storage/Stability

>6 months at –80°C.

Assay Conditions

PLA reaction was performed using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl₂, 0.5 mg/ml BSA, 10 µM substrate and 200 ng PLA2G4A. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Phospholipases

Regulatory Status

Research Use Only